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J.MonicaV.SheoranN.MunjalV.SuseelabhaiR.et al

Here is a practical framework that balances efficiency with safety

Transfer the solution slowly along the vial wall to support controlled mixing and minimize unnecessary agitation of the lyophilized material

In other rodent work, subcutaneous administration has been used, particularly for systemic-effect studies

Individuals with pre-existing health issues such as liver disease, kidney problems, or those undergoing chemotherapy should be especially cautious