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The glutathionylated/oxidized enzymes were again purified via Ni-NTA affinitiy chromatograpghy followed by desalting on a PD-10 column as described above

Given that GABA functions to reduce neuronal excitability in the CNS, the NHPN-1010induced enhancement of 1 subunit expression in the DCN could indicate a reversal of central neural hyperactivity

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