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The primary inoculum was prepared by transferring 1 mL of the stock culture to 30 mL of LB medium in a 250 mL flask and culturing at 37 C for 6 h

DOI: 10.1002/fsn3.2577, dos S

24 Under normal circumstances, to preserve high inuacellular concentrations of free GSH, GSSG is reduced rapidly back to GSH by the NADPH-dependent GSSG reductase

It doesnt occur naturally in the body but is derived from a partial sequence found in human gastric juices