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After incubation, mitochondria were pelleted (1 min at 16,000 g ) and pellets were resuspended in 200 L of 50 mM HEPES buffer (pH 7.4) supplemented with 10 mM IAM-TPP, disrupted using mechanical micro-homogeniser and sonicated for 5 min

10.3389/fmed.2020.629413 Front Med