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Caspase-3 Cleavage Analysis by Western Blotting Cells were scraped and lysed in buffer containing 10 mM Tris HCl (pH 7.5), 150 mM NaCl, 1% Nonidet P-40, 1 mM ethylenediaminetetraacetic acid (EDTA), and 1 mM ethylene glycol tetraacetic acid (EGTA)

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