The lysate was centrifuged at 13000 g at 4 C, and supernatant subjected to deproteinization procedure via TCA precipitation (Abcam
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If you need a more concentrated solution, you could use less bac water
The difference between treated and untreated injury recovery often becomes apparent during this window
Draw the desired amount of BAC water into an insulin syringe, insert the needle through the vials rubber stopper, and slowly release the water along the inside wall of the vial
However, metformin administration failed to rescue paw withdrawal thresholds or pain sensitivity in Nrf2 KO mice, unlike the protective effects observed in MIA-induced WT OA mice (Fig