The beads were washed three times for 5 min each with low-salt wash buffer containing 20 mM Tris-HCl, 1% Triton X-100, 2 mM EDTA, 150 mM NaCl, and 0.1% SDS
NobelPrize.org
Importantly, ferroptosis has been proven to be a common intervention target for halting DKD progression (187)
"Cyanocobalamin reductase / alkylcobalamin dealkylase (MMACHC, Human)"
Warning: As with any supplement, consult your health professional before use if you are pregnant, breastfeeding, or trying to conceive, or if you are taking medication, have a medical condition, or anticipate a surgery
glutamate, cysteine and glycine