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Non-specific antibody binding was reduced by incubating in 10% donkey serum (Sigma-Aldrich, UK) diluted in PBS for at least 30 min, followed by simultaneous incubation in primary antibodies (a melanocyte marker with a target antibody
The main difference is how many hormone pathways each drug targets
Deciphering the reversible glutaredoxin-dependent redox reactions of roGFP2 therefore not only closes a gap in our knowledge of glutaredoxin catalysis but also facilitates the intrepretation of roGFP2 responses from redox measurements and mechanistic analyses in vivo
Shub A., Miranda M., Georgiou H.M., et al
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