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The final reaction mixtures (100 l) contained 2 M His-roGFP2 and 2 M of the respective strep-MBP-GRX fusion proteins

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Thus, the crucial equilibrium between ROS production and antioxidant reserve is vital to ensure cellular redox homeostasis and general well-being [29]

Simultaneously, ROS induce oxidative stress, which can lead to lipid peroxidation, protein oxidation, and DNA damage