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The tube was centrifuged for 5 min at maximum speed in a microcentrifuge (~16,000 g ), and the supernatant (cytoplasmic extract) was transferred to a tube

Protocol phase also matters

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The Nrf2-Keap1 pathway is another key regulator, turning on genes that drive glutathione production and detoxification

This helps add to the effects or to raise GHK-Cu levels when they have dropped