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6 Mechanisms of dysbiosis-induced effects on insulin resistance and -cell function Alterations in the composition and functional activity of the gut microbiota (dysbiosis) are considered a significant factor in the pathogenesis of both T2D and type 1 diabetes (T1D)

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Tissue sections were permeabilized with 0.5% Triton X-100 for 20 min and then blocked with 5% goat serum for 30 min at room temperature

In this report we demonstrate the efficacy of multiplexing the CellTox Green Cytotoxicity Assay with the GSH/GSSG-Glo Assay to determine both the cytotoxic and oxidative effects of compounds on cultured cells in a single assay plate