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The target protein is eluted by changing the pH or using an excess of Fc-specific peptide

"GLP-1/GLP-1R axis might directly act on ovarian MGCs [mural granulosa cells] to promote the follicular development in a dose-dependent manner, likely contributing to the oocyte maturation in PCOS." G

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The orexigenic action of ghrelin is mediated through direct stimulation of the orexigenic AgRP/NPY neurons and concomitant inhibition of the anorectic POMC/CART neurons in the ARC (143, 144)

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