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Collectively, these findings suggest that the future therapeutic strategy may shift from single pathway blockage to multi-target homeostasis restoration, thereby enhancing clinical efficacy
This assay has been validated for human whole blood, serum, EDTA and heparin plasma, urine, and isolated erythrocytes
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For fluorescence staining, we incubated samples for 2 h with Alexa-488 and Alexa-594 conjugated donkey antibodies (1:200) against mouse and rabbit immunoglobulins (Supplementary Figs 6 and 7 are representative images from two sets of immunolabelled cultures of NPCs and neurons)
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