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Add 300 L complete medium and 300 L JC-10 staining working solution, mix well and incubate in a incubator for 20 min, then discard the working solution and wash twice with 1x JC-10 staining buffer, followed by 600 L cell culture medium
Slmmng
Keep the vial in the main refrigerator compartment, not the door where temperature fluctuates
It occurs exclusively in the cytosol, where glutamate cysteine ligase (GCL) and glutathione synthetase (GS) perform their function
The volume of water you add affects the concentration of the peptide solution
This article compares the three from a research perspective
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