10.3389/fgene.2024.1504114 47 HuangM.ChengS.LiZ.ChenJ.WangC.LiJ.et al (2024)
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the solution should preferably have a pH of about 6 to 9, preferably 6,5 to 7,5
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Blots were incubated with HRP-conjugated secondary antibodies (BS13278, Bioworld Technology Inc., MN, USA) for 1 h, and protein expression was detected with ECL (WBULS0500, Millipore, MA, USA) by digital imaging systems (SYNGENE, Cambridge, UK)
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