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Fully reduced protein was incubated with indicated concentrations of APR-246 for one hour at 37 C and desalted with Amicon Ultra Centrifugal Spinners with 3000 cutoff for 30 min at 14000 g to remove excess compound

JNK, c-Jun N-terminal kinase

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Oxidative stress is a type of cellular damage resulting from an imbalance between the production of potentially harmful molecules, called reactive oxygen species (ROS), and the cells ability to clear them with antioxidants