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In addition to physical exertion, fatigue can be brought on by conditions like arthritis, anemia, fibromyalgia, chronic fatigue syndrome, colds, flu, eating disorders, sleeping problems, autoimmune disorders, and diabetes

The protein concentration was quantified using a BCA kit, and then SDS-PAGE buffer was added

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Additionally, the study observed an elevation in superoxide dismutase (SOD) levels, ultimately alleviating obesity-related lung inflammation (Andr et al., 2016)